Harper, James T.

The Strategic Plan has been accepted for inclusion in <a href="https://digitalcommons.unl.edu/birdstrike2009/">2009 Bird Strike North America Conference</a> (11th Joint meeting of Bird Strike Committee USA & Canada, Victoria BC, Canada, 14-17 September 2009). <p> The international aviation community recognizes the high human and economic costs associated with bird strikes. Hundreds of lives and millions of dollars have been lost in recent years because of this problem. Notably, aviation experts in North America recognize the importance and availability of potential solutions for this problem. Several models and systems such and the USAF’s Bird Avoidance Model (BAM) and the Avian Hazard Advisory System (AHAS) as well as the technological development of advanced radar and communications systems have made great progress in addressing the problem of bird strikes. However, many have argued that further and much greater advancement could be made if the current fragmented and competitive efforts could be consolidated in a single cooperative venture. This strategic plan is the initial step in a process of consolidating and integrating the various United States and Canadian civil and military efforts in order to develop and implement North American Bird Strike Advisory System. The plan has been developed based on the collected wisdom and technical knowledge of the top personnel and organizations in the field of aviation safety. If implemented, the plan will represent a critical first step leading to the realization of a North American Bird Strike Advisory System that will help protect aviators and their equipment from the deadly and costly effects of bird hazards. The plan outlines the architecture of a notional bird strike advisory system for North America. It identifies the key agencies that must be involved in the development of the system. It establishes a top level schedule and identifies six key goals in developing an integrated system. The plan describes more detailed objectives and activities required to accomplish these goals. Recommendations are made regarding which agencies might most effectively take the lead in integrating various activities needed to accomplish each goal. It proposes a 5 year budget of approximately $16,000,000 in order to support the initial phases of the effort. The strategic plan and its appendices also outline in considerable detail the key technical challenges, risks, and suggested organizational and technological solutions for these problems. While reviewing this strategic plan, it’s important to remember that it is not a detailed blueprint for developing and implementing the final system. Rather, it is a starting point for an evolving project and system that can be continuously developed and improved as technology and organizational systems become more advanced. The relatively modest budget proposed is essentially a “down payment” for the more robust system that will evolve based on this initial consolidation and integration effort. The plan represents an important first step in moving beyond fragmented competitive approaches to consolidated and integrated system that will save hundreds of lives and prevent a great deal of economic loss associated with destroyed or damaged aircraft and equipment. The plan highlights the many advantages of an integrated and consolidated bird strike advisory system. One such advantage is the improvement in the accuracy and fidelity of bird avoidance information to users in the aviation community. Another advantage of the proposed system is the synergistic use of data from new and existing radar and other systems to enhance reporting on bird activity without compromising the current effectiveness of those systems. Throughout the plan, the development of a robust communications infrastructure and network is described to enhance the timeliness and scope of bird advisory information delivery.

Small-subunit ribosomal DNA (SSU) sequences for 14 members of the rhodophyte order Acrochaetiales were used to generate phylogenetic trees to determine whether the Acrochaetiales is monophyletic and to assess the relationships of acrochaetioid algae to closely related taxa. Within the Acrochaetiales, two strongly supported groups are recognized: one group that includes, in addition to three other species, the type species of the genera Acrochaetium, Audouinella and Rhodochorton; and a second group that includes an unequivocal representative of Colaconema and nine additional species. The relationships of these groups with the Batrachospermales, Palmariales and Nemaliales were not resolved and the possibility that the Acrochaetiales is polyphyletic was not eliminated. Resolution of relationships for species within these groups was strong in only a few cases, with many of the phylogenetic issues at this level remaining equivocal. Our results do not position Camontagnea in the Acrochaetiales, as has recently been suggested, but strongly ally this genus to the family Rhodothamniellaceae of the Palmariales. The phylogenetic position of Rhododraparnaldia, an alga intermediate between the Acrochaetiales and Batrachospermales, remains unresolved. These molecular results provide a foundation for assessing the taxonomic significance of phenotypic characters (e.g. anatomy, life histories, morphology, phycoerythrin type and plastid details) variously considered taxonomically significant in the Acrochaetiales.

Systematics of the red algal order Acrochaetiales and related taxa was investigated using combined small- and large-subunit nuclear ribosomal DNA (SSU and LSU rDNA, respectively) sequence data. These data were subjected to distance, parsimony and maximum likelihood analyses. The resulting phylogenies were congruent with previously published SSU results in that all included orders (Balbianiales, Batrachospermales, Nemaliales, Palmariales and Thoreales) were resolved as monophyletic except the Acrochaetiales, which consisted of two lineages (Acrochaetiales I and II). The Batrachospermales and Thoreales occupied equivocal positions as early diverging lineages, while the Balbianiales generally resolved as sister to an Acrochaetiales–Nemaliales–Palmariales (ANP) complex. Relationships among the four lineages of the ANP complex were not completely resolved, but detailed analyses weakly positioned Acrochaetiales II as sister to the Nemaliales, whereas Acrochaetiales I displayed a moderate to strong affiliation with the Palmariales. Acrochaetiales I included representatives of the genera Acrochaetium, Audouinellaand Rhodochorton, whereas Acrochaetiales II had a number of acrochaetioid species including a representative of the genus Colaconema. Compared with published SSU phylogenies, bootstrap values within the two Acrochaetiales lineages increased substantially in combined SSU/LSU analyses. Based on these results, emended generic descriptions are provided for Acrochaetium, Audouinella, Colaconemaand Rhodochorton, and a new family of acrochaetioid algae is described, the Colaconemataceae J. T. Harper etG. W. Saunders. The Acrochaetiaceae now includes the genera Acrochaetium, Audouinellaand Rhodochorton, while the Colaconemataceae is considered monogeneric at this time. It is quite likely that additional genera will be recognized within the Colaconemataceae pending further investigation. Acrochaetiaceae is retained as the sole family of the Acrochaetiales, although the tenuous recognition of the Palmariales as distinct from this order is discussed, whereas Colaconemataceae is transferred to the new order Colaconematales J. T. Harper et G. W. Saunders.

Background: Lateral gene transfer is increasingly invoked to explain phylogenetic results that conflict with our understanding of organismal relationships. In eukaryotes, the most common observation interpreted in this way is the appearance of a bacterial gene (one that is not clearly derived from the mitochondrion or plastid) in a eukaryotic nuclear genome. Ideally such an observation would involve a single eukaryote or a small group of related eukaryotes encoding a gene from a specific bacterial lineage. <p>Results: Here we show that several apparently simple cases of lateral transfer are actually more complex than they originally appeared: in these instances we find that two or more distantly related eukaryotic groups share the same bacterial gene, resulting in a punctate distribution. Specifically, we describe phylogenies of three core carbon metabolic enzymes: transketolase, glyceraldehyde-3-phosphate dehydrogenase and ribulose-5-phosphate-3-epimerase. Phylogenetic trees of each of these enzymes includes a strongly-supported clade consisting of several eukaryotes that are distantly related at the organismal level, but whose enzymes are apparently all derived from the same lateral transfer. With less sampling any one of these examples would appear to be a simple case of bacterium-to-eukaryote lateral transfer; taken together, their evolutionary histories cannot be so simple. The distributions of these genes may represent ancient paralogy events or genes that have been transferred from bacteria to an ancient ancestor of the eukaryotes that retain them. They may alternatively have been transferred laterally from a bacterium to a single eukaryotic lineage and subsequently transferred between distantly related eukaryotes. <p>Conclusion: Determining how complex the distribution of a transferred gene is depends on the sampling available. These results show that seemingly simple cases may be revealed to be more complex with greater sampling, suggesting many bacterial genes found in eukaryotic genomes may have a punctate distribution.