We examined hematological parameters in four related sciurid species in the late summer–autumn to assess the role of habitat, elevation, body size, and behavior in shaping these parameters. Red squirrels (Tamiasciurus hudsonicus) and Arctic ground squirrels (Spermophilus parryii) were sampled in southwestern Yukon, yellow-pine chipmunks (Tamias amoenus) in southern Alberta, and the eastern grey squirrel (Sciurus carolinensis) in southern Ontario. We obtained whole blood samples from each species and compared glucose levels, red blood cell characteristics (hematocrit, red blood cell count, hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration), and white blood cell counts (neutrophils, lymphocytes, monocytes, eosinophils, basophils) across species. We found species differences in glucose and red blood cell characteristics that may be a function of activity levels, phylogeny, or elevation, but not of body size, body condition, or adaptations to a semi-fossorial habitat. We also found species differences in white blood cell counts that remain unexplained by any single simple explanation and may be more useful for comparison of individuals within a given species than for interspecies comparisons.
Virtually all biological characteristics of organisms change with age, and thus, to assess the impact of these changes, accurate aging techniques are essential. However, many current methods are unable to accurately distinguish among adults of different ages. We determined the age of yellow-pine chipmunks (Tamias amoenus) from the Rocky Mountains of Alberta using eye lens masses, annuli from mandible sections, and annuli from femurs. Each of these methods was assessed against nine known-age animals and seven animals that had not been caught previously and were presumed to be juveniles. Eye lens masses could distinguish juveniles from adults but not adults of different ages. Mandibular sections were not practical in this species because of excessive tearing during sectioning. Femoral sections precisely predicted age. We found that the number of adhesion lines, minus one, accurately represented the ages of adults ranging from 1 to 5 years old. Femoral annuli have not previously been used to age mammals and our results suggest that they may be useful in aging other mammals, especially rodents.
